Prep HPLC Column, HPS Silica, 5um, 120A. 21.2mm ID x 150mm Length. Cogent Brand. 1 EA.
Additional Info:
75001-P150 Cogent HPS Silica™ Prep HPLC Column is packed with high‑purity, unmodified silica, delivering consistent and reproducible performance for preparative purification of polar and hydrophilic compounds.
Because the stationary phase is unbonded and unmodified, it provides strong surface silanol interactions, making it highly effective in:
Normal Phase chromatography (primary mode)
HILIC separations for polar analytes (highly suitable secondary mode)
This dual‑mode capability allows maximum flexibility in method development, enabling separations using either non‑polar systems (NP) or high‑organic mobile phases (HILIC).
This proprietary HPS Silica™ has been widely used in HPLC laboratories for many years as a premium-value alternative to columns such as Inertsil® and Luna®, offering reliable selectivity and reproducibility.
The 5 µm particle size is optimized for preparative use, providing:
Lower backpressure vs smaller particles
Higher flow rate capability
Robust performance for repeated purification cycles
150 mm Length – High Resolution Preparative Separations
The 150 mm length provides enhanced resolving power for more demanding separations.
Higher resolution than 50 mm and 100 mm preparative columns
Improved separation of closely related compounds
Increased peak capacity for complex mixtures
✅ Recommended for purification workflows requiring improved resolution and higher purity outcomes
21.2 mm ID – Preparative Scale
Designed for preparative HPLC purification
High sample loading capacity
Suitable for scale‑up from analytical and semi‑prep methods
Compatible with large‑volume injection and collection systems
✅ Recommended for process development and production‑scale purification
Flow Rate & System Requirements (21.2 mm ID, 5 µm)
⚠️ Requires preparative or process‑scale HPLC system capability
Typical flow rate range: ~15 – 40 mL/min
Moderate backpressure (increased with 150 mm length)
Designed for high‑flow purification workflows
Compatible with preparative detectors and fraction collectors
✅ Important: A specialized preparative HPLC system is required for columns of this size
Why Choose HPS Silica™ for Preparative HPLC
Unmodified Silica – Dual Mode Capability
Ideal for Normal Phase separations
Highly suitable for HILIC separations of polar compounds
High‑Purity Silica Surface
Strong retention of hydrophilic analytes
Consistent and reproducible selectivity
USP L3 Compatibility
Suitable for compendial and validated methods
Reliable alternative to legacy silica phases
Proven Performance
Widely used across analytical and preparative laboratories
Long‑term, dependable results
Performance Advantages
Low Backpressure (5 µm Particles)
Enables higher flow rates for preparative purification
Compatible with standard preparative systems
Fast Equilibration
Reduces cycle time between runs
Flexible Selectivity
Supports both Normal Phase and HILIC method development
Typical Applications
Preparative purification of fats and lipids
Polar compound isolation
Normal phase and HILIC separations
Process scale‑up from analytical HPLC
Pharmaceutical and chemical purification workflows
Important Usage Notes
Suitable for both Normal Phase and HILIC chromatography conditions
Use appropriate non‑polar solvents (NP) or high‑organic mobile phases (HILIC)
Requires preparative or process‑scale HPLC system
Ensure proper equilibration for reproducible retention.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable